多克隆抗体定制服务

       多克隆抗体能够识别多个抗原表位，并且由于制备周期短，成本低，应用广泛等优点已成为科研人员进 行免疫学研究不可或缺的重要工具。戴安生物凭借5000+多抗项目研发和生产经验，可以提供兔、大鼠、小鼠、豚鼠、 羊、鸡等多个宿主在内的多抗制备。并且已成功定制过以下多种属蛋白质抗体：人、小鼠、大鼠、斑马鱼、银鲫、草鱼、 黄颡鱼、鳜鱼、海参、海胆、线虫、、四膜虫、细菌、病毒、拟南芥、蓝藻、棉花、玉米、水稻、马铃薯、油菜、小麦等，实战经验非常丰富，提供多个物种，多种抗原，多个应用平台的多克隆抗体定制，能够最大程度保证抗体定制的成功率。

• 免费分析抗原序列，利用专业的抗原设计软件设计抗原，结合文献，综合选择免疫原序列
• 提供优质重组蛋白质抗原（原核+真核），用于免疫的抗原（上清表达成功率>90%），纯度>80%，浓度 >1mg/ml
• 合成高纯度多肽抗原，提供多肽质检报告；小分子抗原采用独特的活化和偶联方案
• 利用自主研发佐剂，保证抗原和佐剂充分混匀，多点皮下注射，最大程度上提高动物的血清效价
• 构建第二套载体表达蛋白，用于纯化血清，减少标签蛋白对抗体特异性的影响，纯化出高特异性抗体

• 客户进行Western Blot内源性或者IP过表达检测小样后能够检测到目的条带（说明抗体验证合格）；
• Western Blot内源性或者IP过表达检测阴性无法满足实验需要，说明此抗体订单验证失败，则甲方无需再付此项目剩余尾款；
• 间接ELISA检测抗血清效价，针对免疫原效价，兔多抗不低于1:160,000，小鼠多抗不低于1:6,4000；
• 客户有权随时了解服务项目进展情况，并对成果拥有全部所有权。

[1] Xiaobo Yu,et al. Fertilized egg cells secrete endopeptidases to avoid polytubey. Nature. 31 March.2021

[2] Zhaojing Lu ,et al. Deletion of the transmembrane protein Prom1b in zebrafish disrupts outer-segment morphogenesis and causes photoreceptor degeneration .JBC. July 30, 2019   

[3] Meng-Jie Zhao,et al. MiR-15b and miR-322 inhibit SETD3 expression to repress muscle cell differentiation [J]. Cell Death & Disease. 22 February 2019

[4] Miao Li1,2,3*, Tao Huang1,2,3*, Meng-Jing Li1,2,3*, et al. The histone modification reader   ZCWPW1 is requiredfor meiosis prophase I in male but not in female mice [J].

Science Advances 14 Aug 2019: Vol. 5, no. 8, eaax1101

[5] Functional characterization of IL-10 and its receptor subunits in a perciform fish, the mandarin fish, Siniperca chuatsi [J]. Developmental & Comparative Immunology.

Volume 97, August 2019, Pages 64-75

[6] Yao Wang1 ,et al. Mitochondria-localised ZNFX1 functions as a dsRNA sensor to initiate antiviral responses through MAVS [J].Nature Cell Biology 2019 Nov;21(11):1346-1356. 

[7] Linlin Shi 1, Weimin Wang 1 and Yanhe Li 1,4,*et al. Molecular Characterization and Functional Study of Insulin-Like Androgenic Gland Hormone Gene   in the Red Swamp Crayfish, Procambarus clarkii[J]. MDPI Genes 2019

[8] Jing Hou, Shan Nan Chen, Zhen Gan, Nan Li, Lin Huang, Hui Jun Huo, Yue Cong Yang, Yishan Lu, Zhan Yin and Pin Nie   In Primitive Zebrafish, MHC Class II Expression Is Regulated by IFN-γ, IRF1, and Two Forms of CIITA   J Immunol 2020 May 1;204(9):2401-2415

[9]JunyanTao, Chenglian Bai, Yuanhong Chen, Huanhuan Zhou, Yahui Liu, Qingyu Shi, WenhaoPan, Haojia Dong, Luyi Li, Hui Xu, Robyn Tanguay, Changjiang Huang, QiaoxiangDong   Environmental relevant concentrations of benzophenone-3 induced developmental neurotoxicity in zebrafish[J].Science of The Total Environment 2020

[10]Qiannan Zhang1 , Yanghong Xu1, Jishuai Huang1, Kai Zhang2, Haijun Xiao3 8 ,XiaojianQin4, Linlin Zhu5, Yingguo Zhu1,#, and Jun Hu1*   Therice pentatricopeptide repeat protein PPR756 is involved in pollen developmentby affecting multiple RNA editing in mitochondria.  Front Plant Sci 2020 Jun 12;11:749

[11] Xinfeng Li et al.  Clp protease and antisense RNA jointly regulate the global regulator CarD to mediate mycobacterial starvation response  Elife. 2022 

[12] Xinfeng Li et al.  Ccdc38 is required for sperm flagellum biogenesis and male fertility in mouse  Biorxiv January 11, 2022.