磷酸化抗体定制服务
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服 务 简 介 | |||||||||||||||||
戴安生物凭借多年经验和技术积累,提供位点修饰性抗体定制服务,制备的修饰性位点特异性抗体,不识别非修饰性抗原 , 具有高度的特异性和灵敏度,且保证针对免疫原效价不低于1:160,000;与非修饰性多肽的交叉反应<10%性抗体定制。可提供多种修饰性抗体服务包括:
戴安生物同时提供翻译后修饰泛抗体系列产品:赖氨酸乙酰化,甲基化,琥珀酰化和丙二醛-甲醛化等泛修饰特异性抗体。
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服 务 内 容 |
服务步骤
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项目内容 |
周期 |
交付内容 |
报价 |
抗原分析及设计 | 根据客户指定修饰位点,分析蛋白免疫原性,亲水性,同源性等选择合适的特异性多肽序列 | 2个工作日 | 分析报告 |
询价 027-59325199
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抗原制备 | 对特定的蛋白结合位点序列设计合成修饰性多肽,并与KLH偶联用于免疫 | 3-4周 |
1mg修饰性多肽 |
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合成对应的非修饰性多肽,偶联BSA用于纯化 | 1mg非修饰性多肽 | |||
动物免疫 | 免疫4只兔子,每只宿主进行5次免疫,包括初次免疫(加入完全弗氏佐剂)和4次加强免疫(加入不完全弗氏佐剂) | 65天 | 兔血清60ml(其他宿主另计) | |
效价检测 | 检测血清ELISA效价,将免疫原包被进行ELISA检测 | 2个工作日 | 效价检测结果 | |
抗体纯化 | 用修饰性多肽和非修饰性多肽进行交叉吸附纯化 | 7个工作日 | 10ml抗血清的亲和纯化抗体5mg | |
效价检测 | 检测抗体ELISA效价,将免疫原,吸附抗原分别包被进行ELISA检测,检测检查吸附效果 | 2个工作日 |
效价检测结果 项目报告 |
服 务 流 程 | |||||
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发 表 文 献 (部 分) |
[1] A natural DNMT1 mutation elevates the fetal hemoglobin level via epigeneticderepression of the γ-globin gene in β-thalassemia
Blood, May 25, 2021, Impact Factor: 22.1
[2] Fructose-1, 6-bisphosphatase 1 interacts with NF-κB p65 to regulate breast tumorigenesis via PIM2 induced phosphorylation
Theranostics, Oct 19, 2020,
[3] Primary tumor‐secreted VEGF induces vascular hyperpermeability in premetastatic lung via the occludin phosphorylation/ubiquitination pathway
R Li, et al. Molecular Carcinogenesis 2019 Dec;58(12) |
[4] Comparative proteomic analysis of histone modifications upon acridone derivative 8a-induced CCRF-CEM cells by data independent acquisition
Y Wang et al. Journal of proteome
[5] The male germline-specific protein MAPS is indispensable for pachynema progression and fertility
Miao Li et al PNAS February 23, 2021 118 (8) e2025421118;
[6] PaACL silencing accelerates flower senescence and changes the proteome to maintain metabolic homeostasis in Petunia hybrida
H Zhao et al. Journal of Experimental Botany
[7] Inhibition of PLA2G4E/cPLA2 promotes survival of random skin flaps by alleviating Lysosomal membrane permeabilization-Induced necroptosis
Junsheng Lou et al. Zool Res, 2021
[8] O-GIcNAcylation stabilizes the autophagy-initiating kinase ULK1 by inhibiting chaperone- mediated autophagy upon HPV infection.Yingxin Shi et
al.Journal Of Biological Chemistry,2022
[9] Global proteome analyses of phosphorylation and succinylation of barley root proteins in response to phosphate starvation and recovery.Juncheng Wang et al.Frontier in Plant Science,2022
[10] IL-1b-associated NNT acetylation orchestrates ironsulfur cluster maintenance and cancer immunotherapy resistance.Yi Han et al.Molecular Cell,2023
[11] p38-mediated FOXN3 phosphorylation modulates lung inflammation and injury through the NF-κB signaling pathway.Xinxing Zhu et al.Nucleic Acids Research, 2023
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